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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 9;188(10):4876–4884. doi: 10.4049/jimmunol.1200389

Figure 4. PD-1 deficient CTLs are more effective killers of cardiac-derived target cells, in vitro.

Figure 4

Naïve OT-1+ CD8+ T cells were isolated from Pd1−/− or WT mice, and cultured for 5 days in the presence of IL-2 and IL-12 to generate effector cells, and then rested overnight. Confluent mouse heart endothelial cells (MHEC) were pre-treated with IFNγ for 2 h and pulsed with the ovalbumin peptide SIINFKL. The MHEC were then co-cultured with OT-1 effectors at the indicated ratios of effector to target cell ratios for 1 h, and were then analyzed by FACS for Annexin V and 7-AAD staining (A). Supernatants from these assays were analyzed for the presence of IL-2 (B) and IL-10 (C) using multiplex luminex-bead based cytokine assays, and IFNγ (D) and granzyme B (E) by ELISA. Data presented represent the mean ± S.E.M of 3 experiments. * P<0.05, ** P<0.01 *** P< 0.001