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Important positions used to calculate the SH3-peptide MIECs. Asterisks in the first line of the multiple sequence alignments show the important positions for (a) the class-I SH3 domains and (b) the class-II SH3 domains. The alignments are colored according to the consensus sequence conservation (conservation larger than 20%) using the ClustalX coloring scheme.69 The figures were generated using Jalview.70
