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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Bioorg Med Chem. 2012 Mar 29;20(10):3298–3305. doi: 10.1016/j.bmc.2012.03.043

Figure 3. Elution of pig mammary gland protein from ATP-Sepharose resin with SNX-2112, PU-H71 and Ligand construct 8.

Figure 3

ATP resin was charged with pig mammary gland extract and washed in bulk. Then, 20μl of charged resin was placed in each well of a 96-well filter plate and eluted with the indicated compounds in parallel. The eluates were characterized by SDS-PAGE, silver staining and mass spectrometry.