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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 13;188(10):4992–5002. doi: 10.4049/jimmunol.1101992

FIGURE 5.

FIGURE 5

YY-1 binds to the p18 promoter in a C/T allele dependent fashion. Representative EMSAs performed with B6 (A) or B6.Sle2c1 (B) spB cell nuclear extracts showing DNA-protein complexes (bands 1 and 2) captured more strongly by C than the T allele biotin-labeled oligonucleotide probes. Specificity of this interaction was demonstrated by adding increasing amounts of unlabeled “cold” C or T-allele oligonucleotides as competitors or anti-YY-1 Ab. The arrow on the right of lane 12 indicates the supershift created by the anti YY-1 Ab. The content of each lane indicated in the table is common to both types of nuclear extracts.