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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 9;188(10):5086–5093. doi: 10.4049/jimmunol.1102914

Figure 2.

Figure 2

C5a mediates inhibition of IL-27(p28) from TLR4-activated macrophages. (A) ELISA of IL-27(p28) levels in supernatants from macrophages after incubation with LPS alone, the combination of LPS and different concentrations of recombinant mouse C5a (100-10 nM) or C5a alone (100 nM), 10 h.(B) Suppression of IL-27(p28) production from LPS-activated macrophages by co-incubation with C5a (100 nM) at different time points. (C) RT-PCR of mRNA levels for IL-27(p28) in macrophages after LPS alone or in combination with C5a (100 nM). (D) Effects of recombinant mouse C5adesArg (100 nM) on IL-27(p28) secretion by LPS-activated macrophages, 10h. (E) IL-27(p28) release from macrophages after co-stimulation with LPS and formyl-methionyl-leucyl-phenylalanine (FMLP, 4 µM) compared to LPS alone, n.s. denotes not significant. All experiments shown were done with PEM from C57BL/6J (Wt) mice and LPS was used as 1 µg/ml. Data are representative of at least 3 independent experiments. *p < 0.05, error bars represent s.e.m.