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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 9;188(10):5086–5093. doi: 10.4049/jimmunol.1102914

Figure 3.

Figure 3

C5a suppresses IL-27(p28) via the C5aR but not the C5L2 receptor. (A) Flow cytometry analysis of the C5aR receptor together with the macrophage surface marker F4/80 on PEM from Wt mice or C5aR−/− mice. (B) PEM were left as resting controls (Ctrl) or stimulated with LPS (1 µg/ml) for 12 h, both in the presence of Monensin (2 µM). Cells were then analyzed for F4/80, C5aR and intracellular IL-27(p28) by flow cytometry. Dot-plots were gated to show only F4/80+ events. (C) PEM of the strains C57BL/6J, C5aR−/− and C5L2−/− were incubated with LPS and increasing concentrations of C5a (0, 10, 100 nM) for 10 h. (D) Relative inhibition of LPS-induced IL-27(p28) release in macrophages from C57BL6/J, C5aR−/− or C5L2−/− mice by C5a (100 nM). Levels of IL-27(p28) with LPS alone were used as 100% for each individual strain. All experiments were done with thioglycollate elicited macrophages (PEM) and LPS was used as 1 µg/ml. Data are representative of at least 3 independent experiments. *p < 0.05, error bars represent s.e.m.