Table 1.
Serine Hydrolase, IC50 (nM)** | |||
---|---|---|---|
Oxon | Carboxylesterase 1 | Carboxylesterase 2 | Monoacylglycerol lipase |
Chlorpyrifos Oxon | 0.15 ± 0.05a | 0.33 ± 0.05a | 5.1 ± 3.7b |
Paraoxon | 0.38 ± 0.10a | 6.2 ± 0.3b | 120 ± 40c |
Methyl Paraoxon | 4.8 ± 0.2b | 220 ± 50c | 1600 ± 960d |
All reactions were carried out at 37°C with enzyme and inhibitor preincubated for 15 min prior to the addition of substrate.
Values are means ± standard deviation of at least 3 independent experiments. All values in the same row or column with different superscripts are significantly different (p< 0.001, ANOVA and Tukey’s post-hoc test).