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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Cell Rep. 2012 Mar 29;1(4):385–391. doi: 10.1016/j.celrep.2012.02.008

Table 1.

Analysis of embryonic lethality due to loss of miRNAs.

line +/+:Cre −/−:Cre Total # of pups lethality
miR-7a-2 8 (5) 7 (5) 41 no
miR-30b/30d 7 (5) 6 (5) 39 no
miR-130a 6 (5) 4 (5) 42 no
miR-141/200c 4 (5) 4 (5) 43 no
miR-146a 4 (4) 3 (4) 33 no
miR-205 8 (7) 0 (7) 56 yes
miR-210 5 (5) 5 (5) 40 no
miR-296/298 4 (5) 4 (5) 39 no
miR-497/195 11 (5) 4 (5) 41 no
miR-654/376b 8 (5) 3 (5) 43 no
miR-688 6 (5) 6 (5) 40 no

To determine if a miRNA gene (or a cluster) is essential during development, homozygous lacZ-KO mice were generated by intercrossing lacZ-neo-flox/+ and lacZ-KO/+: actin-Cre transgenic mice. About 40 offspring were genotyped and the expected number of animals was indicated inside parenthesis. +/+:Cre denotes wild type/wild type with Cre and −/−:Cre indicates lacZ-KO/lacZ-KO with Cre.