Fig. 6.

Integrin-mediated engagement of Fak, Src and PI3-K/Akt-1 in HIEC cell survival and suppression of anoikis. a Representative (n ≥ 3) WB analyses of Akt-1, Src and Fak IPs from HIEC cell control cultures or cultures treated with PF573228, PP2, non-immune IgGs, the β1 integrin-blocking antibody P4C10, or kept in suspension in polyHEMA-coated dishes (suspension). Specific antibodies for ^pS473p57^Akt-1, ^pY418p60^Src and ^pY397p125^Fak, as well as for respective total protein forms, were used. b HIEC cells were maintained as in (a), except that the relative activation levels of Fak (open columns), Src (grey columns) and Akt-1 (filled columns) were established, then compared to controls. Statistically significant (0.0005 ≤ P ≤ 0.005) differences between treated and control cultures are indicated by (*)