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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Dev Dyn. 2009 Jun;238(6):1412–1421. doi: 10.1002/dvdy.21932

Figure 2. Evidence that xCARs function as adenovirus receptors.

Figure 2

The lower panels show GFP fluorescence in the cells, the upper show flow cytometry of the above-threshold cells by GFP intensity (vertical axis) and cell size (horizontal axis). The number of GFP positive cells in the presence of xCAR is similar to the transfection efficiency (about 5%), indicating that all or most cells transfected with xCAR become infected. All cell nuclei are stained blue with DAPI.

A. CHO cells treated with lipofectamine alone and infected with adeno-GFP (negative control).

B. CHO cells transfected with pcDNA3-GFP (transfection control).

C. CHO cells transfected with xCAR1, and then infected with adeno-GFP.

D. CHO cells transfected with xCAR2, and then infected with adeno-GFP. Scale bars 5 μm.