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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Dev Dyn. 2009 Jun;238(6):1412–1421. doi: 10.1002/dvdy.21932

Figure 3. Expression of xCAR mRNA in embryonic and tadpole stages.

Figure 3

A. RT-PCR of xCAR1 and xCAR2 in mRNA extracted from whole embryos at the developmental stages indicated. xCAR1 is expressed from gastrulation onwards, while xCAR2 is expressed both maternally and zygotically.

B. RT-PCR of xCAR1 and xCAR2 in mRNA extracted from stage 54-56 tadpole tissues. M muscle, Li Liver, H head, S skin.

C-H. Wholemount in situ hybridizations. C, D show no probe controls at stage 25 and 31; E, F show xCAR1 at stage 25 and 31; G, H show xCAR2 at stage 25 and 31. White arrowheads in G and H indicate expression of xCAR2 in dorsal tailbud and in pronephros and nephric duct, which are greater than seen for xCAR1.