Figure 5.
Effect of 25 μg/mL of thyme extracts S1 and S2 from three different species (Thymus vulgaris, Thymus zygis, and Thymus hyemalis) on the relative quantification (RQ) of TNF-α gene expression (a), IL-1β (b), IL-6 (c), and IL-10 (d) on macrophage-THP-1 cells. Cells were differentiated with (PMA) 100 ng/mL for and afterwards treated as described in Section 3 for 24 hours. Data represent means ± SEM calculated from six independent experiments with 3 replications for each treatment. Statistic Dunnett's multiple comparison test versus Ctrl +oxLDL signification is represented by *P values less than 0.05, significant; **P values less than 0.01, very significant; and ***P values less than 0.001, extremely significant. Statistic Bonferroni multiple comparison test versus Ctrl −oxLDL signification is represented by ♦♦P values less than 0.01, very significant; and ♦♦♦P values less than 0.001, extremely significant. One group was treated with a small concentration of diclofenac (5 μg/mL) as positive standard control.