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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: J Invest Dermatol. 2008 Nov 13;129(4):994–1001. doi: 10.1038/jid.2008.332

Figure 8. TNF-α mRNA stability is not induced after UVB irradiation and IL-1α stimulation.

Figure 8

Keratinocytes were UVB or sham-irradiated and stimulated with IL-1α for 3 h. Actinomycin D (10 μg/ml) was added after 3h. Total RNA was extracted at different time points and TNF-α mRNA levels were determined by northern analysis and Real-time PCR. The half-life was calculated in UVB, and UVB+ IL-1α treated cells.