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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Gene Ther. 2012 Feb 2;20(2):136–142. doi: 10.1038/gt.2012.5

Figure 2. Cationic polymer and lipid transfection reagents do not induce phosphorylation of eIF2α in wild-type cells.

Figure 2

Wild-type MEF cells were treated with the indicated transfection reagent or mock-treated and lysed at indicated time points. (A) Phosphorylation of eIF2α was assessed by western blotting. Representative data from one of two independent experiments is shown. (B) Quantitation of western blot band densities. Values were calculated as the ratio of phosphorylated to total eIF2α and normalized to the values obtained in mock-treated cells at 0.1 hours post-shock. Data displayed is mean ± SEM of n = 2 experiments.