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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Gene Ther. 2012 Feb 2;20(2):136–142. doi: 10.1038/gt.2012.5

Figure 7. Nucleofection reduces translation in a GCN2 and PERK dependent manner.

Figure 7

WT or GCN2−/−/PERK−/− MEF cells were nucleofected and then lysed at the indicated time. Luciferase enzymatic activity was measured as relative light units (RLU). Asterisks indicate P-value <0.05 and daggers indicate P-value <0.001 comparing GCN2−/−/PERK−/− to WT MEFs. Data is representative of 3 independent experiments. (A) Cells were transfected with pCMV-luciferase plasmid 24 hours prior to nucleofection. Luciferase activity was normalized to RLU present in non-nucleofected cells (mock) at the same timepoint. Data displayed is mean ± SEM of four replicate wells. (B) Luciferase mRNA was delivered by nucleofection. Data is mean ± SEM of three replicate wells.