Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1992 Dec 11;20(23):6377–6383. doi: 10.1093/nar/20.23.6377

V-src-induced-transcription of the avian clusterin gene.

Y Herault 1, G Chatelain 1, G Brun 1, D Michel 1
PMCID: PMC334530  PMID: 1475199

Abstract

We have isolated the avian gene T64 corresponding to the mammalian clusterin, on the basis of high accumulation of its template mRNA in cells infected with oncogenic retroviruses. Since the clusterin was shown to have a protective effect against the immune system, its induction by oncogenic viruses is of major biological importance. The unique, short 5 kb-long T64 genomic locus is inactive in normal quail embryo fibroblasts in primary culture whereas it shows a high transcriptional activity after transformation by the Rous sarcoma virus. The 963 bp-long 5' flanking region is sufficient to drive the transcription of the chloramphenicol acetyltransferase reporter gene in a thermodependent manner when a thermosensitive version of pp60v-src is used. Deletion and point mutation analyses of the promoter show that the v-src response requires at least two separate elements: PUR and AP-1, located respectively at positions -167 to -152 and -25 to -19 relative to the single transcription initiation site. In addition, the binding of specific nuclear factors to these responsive elements correlates with the T64 promoter activation.

Full text

PDF
6383

Images in this article

6379Image
on p.6379
6380Image
on p.6380
6381Image
on p.6381
6381Image
on p.6381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angel P., Imagawa M., Chiu R., Stein B., Imbra R. J., Rahmsdorf H. J., Jonat C., Herrlich P., Karin M. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor. Cell. 1987 Jun 19;49(6):729–739. doi: 10.1016/0092-8674(87)90611-8. [DOI] [PubMed] [Google Scholar]
  2. Beddington R. S., Morgernstern J., Land H., Hogan A. An in situ transgenic enzyme marker for the midgestation mouse embryo and the visualization of inner cell mass clones during early organogenesis. Development. 1989 May;106(1):37–46. doi: 10.1242/dev.106.1.37. [DOI] [PubMed] [Google Scholar]
  3. Bergemann A. D., Johnson E. M. The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication. Mol Cell Biol. 1992 Mar;12(3):1257–1265. doi: 10.1128/mcb.12.3.1257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bettuzzi S., Hiipakka R. A., Gilna P., Liao S. T. Identification of an androgen-repressed mRNA in rat ventral prostate as coding for sulphated glycoprotein 2 by cDNA cloning and sequence analysis. Biochem J. 1989 Jan 1;257(1):293–296. doi: 10.1042/bj2570293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bettuzzi S., Troiano L., Davalli P., Tropea F., Ingletti M. C., Grassilli E., Monti D., Corti A., Franceschi C. In vivo accumulation of sulfated glycoprotein 2 mRNA in rat thymocytes upon dexamethasone-induced cell death. Biochem Biophys Res Commun. 1991 Mar 29;175(3):810–815. doi: 10.1016/0006-291x(91)91637-r. [DOI] [PubMed] [Google Scholar]
  6. Birchenall-Roberts M. C., Ruscetti F. W., Kasper J., Lee H. D., Friedman R., Geiser A., Sporn M. B., Roberts A. B., Kim S. J. Transcriptional regulation of the transforming growth factor beta 1 promoter by v-src gene products is mediated through the AP-1 complex. Mol Cell Biol. 1990 Sep;10(9):4978–4983. doi: 10.1128/mcb.10.9.4978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Blaschuk O., Burdzy K., Fritz I. B. Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid. J Biol Chem. 1983 Jun 25;258(12):7714–7720. [PubMed] [Google Scholar]
  8. Brandl C. J., Struhl K. Yeast GCN4 transcriptional activator protein interacts with RNA polymerase II in vitro. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2652–2656. doi: 10.1073/pnas.86.8.2652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Buttyan R., Olsson C. A., Pintar J., Chang C., Bandyk M., Ng P. Y., Sawczuk I. S. Induction of the TRPM-2 gene in cells undergoing programmed death. Mol Cell Biol. 1989 Aug;9(8):3473–3481. doi: 10.1128/mcb.9.8.3473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Buttyan R., Zakeri Z., Lockshin R., Wolgemuth D. Cascade induction of c-fos, c-myc, and heat shock 70K transcripts during regression of the rat ventral prostate gland. Mol Endocrinol. 1988 Jul;2(7):650–657. doi: 10.1210/mend-2-7-650. [DOI] [PubMed] [Google Scholar]
  11. Calzone F. J., Britten R. J., Davidson E. H. Mapping of gene transcripts by nuclease protection assays and cDNA primer extension. Methods Enzymol. 1987;152:611–632. doi: 10.1016/0076-6879(87)52069-9. [DOI] [PubMed] [Google Scholar]
  12. Castellazzi M., Dangy J. P., Mechta F., Hirai S., Yaniv M., Samarut J., Lassailly A., Brun G. Overexpression of avian or mouse c-jun in primary chick embryo fibroblasts confers a partially transformed phenotype. Oncogene. 1990 Oct;5(10):1541–1547. [PubMed] [Google Scholar]
  13. Choi-Miura N. H., Ihara Y., Fukuchi K., Takeda M., Nakano Y., Tobe T., Tomita M. SP-40,40 is a constituent of Alzheimer's amyloid. Acta Neuropathol. 1992;83(3):260–264. doi: 10.1007/BF00296787. [DOI] [PubMed] [Google Scholar]
  14. Dehbi M., Mbiguino A., Beauchemin M., Chatelain G., Bédard P. A. Transcriptional activation of the CEF-4/9E3 cytokine gene by pp60v-src. Mol Cell Biol. 1992 Apr;12(4):1490–1499. doi: 10.1128/mcb.12.4.1490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Duguid J. R., Bohmont C. W., Liu N. G., Tourtellotte W. W. Changes in brain gene expression shared by scrapie and Alzheimer disease. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7260–7264. doi: 10.1073/pnas.86.18.7260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Eddy A. A., Fritz I. B. Localization of clusterin in the epimembranous deposits of passive Heymann nephritis. Kidney Int. 1991 Feb;39(2):247–252. doi: 10.1038/ki.1991.29. [DOI] [PubMed] [Google Scholar]
  17. Evan G. I., Wyllie A. H., Gilbert C. S., Littlewood T. D., Land H., Brooks M., Waters C. M., Penn L. Z., Hancock D. C. Induction of apoptosis in fibroblasts by c-myc protein. Cell. 1992 Apr 3;69(1):119–128. doi: 10.1016/0092-8674(92)90123-t. [DOI] [PubMed] [Google Scholar]
  18. French L. E., Polla L. L., Tschopp J., Schifferli J. A. Membrane attack complex (MAC) deposits in skin are not always accompanied by S-protein and clusterin. J Invest Dermatol. 1992 May;98(5):758–763. doi: 10.1111/1523-1747.ep12499946. [DOI] [PubMed] [Google Scholar]
  19. French L. E., Tschopp J., Schifferli J. A. Clusterin in renal tissue: preferential localization with the terminal complement complex and immunoglobulin deposits in glomeruli. Clin Exp Immunol. 1992 Jun;88(3):389–393. doi: 10.1111/j.1365-2249.1992.tb06459.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Guermah M., Gillet G., Michel D., Laugier D., Brun G., Calothy G. Down regulation by p60v-src of genes specifically expressed and developmentally regulated in postmitotic quail neuroretina cells. Mol Cell Biol. 1990 Jul;10(7):3584–3590. doi: 10.1128/mcb.10.7.3584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Gunji H., Hass R., Kufe D. Internucleosomal DNA fragmentation during phorbol ester-induced monocytic differentiation and G0/G1 arrest. J Clin Invest. 1992 Mar;89(3):954–960. doi: 10.1172/JCI115677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Hartmann K., Rauch J., Urban J., Parczyk K., Diel P., Pilarsky C., Appel D., Haase W., Mann K., Weller A. Molecular cloning of gp 80, a glycoprotein complex secreted by kidney cells in vitro and in vivo. A link to the reproductive system and to the complement cascade. J Biol Chem. 1991 May 25;266(15):9924–9931. [PubMed] [Google Scholar]
  23. Herbomel P., Bourachot B., Yaniv M. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma. Cell. 1984 Dec;39(3 Pt 2):653–662. doi: 10.1016/0092-8674(84)90472-0. [DOI] [PubMed] [Google Scholar]
  24. Imagawa M., Chiu R., Karin M. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell. 1987 Oct 23;51(2):251–260. doi: 10.1016/0092-8674(87)90152-8. [DOI] [PubMed] [Google Scholar]
  25. Jenne D. E., Tschopp J. Clusterin: the intriguing guises of a widely expressed glycoprotein. Trends Biochem Sci. 1992 Apr;17(4):154–159. doi: 10.1016/0968-0004(92)90325-4. [DOI] [PubMed] [Google Scholar]
  26. Jenne D. E., Tschopp J. Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7123–7127. doi: 10.1073/pnas.86.18.7123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Kerr L. D., Miller D. B., Matrisian L. M. TGF-beta 1 inhibition of transin/stromelysin gene expression is mediated through a Fos binding sequence. Cell. 1990 Apr 20;61(2):267–278. doi: 10.1016/0092-8674(90)90807-q. [DOI] [PubMed] [Google Scholar]
  28. Kirszbaum L., Sharpe J. A., Murphy B., d'Apice A. J., Classon B., Hudson P., Walker I. D. Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: a link between the complement and reproductive systems. EMBO J. 1989 Mar;8(3):711–718. doi: 10.1002/j.1460-2075.1989.tb03430.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Lee W., Mitchell P., Tjian R. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements. Cell. 1987 Jun 19;49(6):741–752. doi: 10.1016/0092-8674(87)90612-x. [DOI] [PubMed] [Google Scholar]
  30. Leibovitch I., Buttyan R. Sulfated glycoprotein-2 induced endogenous resistance to ischemia and reperfusion injury in the seminiferous tubules. Am J Reprod Immunol. 1991 Oct;26(3):114–117. doi: 10.1111/j.1600-0897.1991.tb00707.x. [DOI] [PubMed] [Google Scholar]
  31. Mattson M. P. Evidence for the involvement of protein kinase C in neurodegenerative changes in cultured human cortical neurons. Exp Neurol. 1991 Apr;112(1):95–103. doi: 10.1016/0014-4886(91)90118-v. [DOI] [PubMed] [Google Scholar]
  32. May P. C., Lampert-Etchells M., Johnson S. A., Poirier J., Masters J. N., Finch C. E. Dynamics of gene expression for a hippocampal glycoprotein elevated in Alzheimer's disease and in response to experimental lesions in rat. Neuron. 1990 Dec;5(6):831–839. doi: 10.1016/0896-6273(90)90342-d. [DOI] [PubMed] [Google Scholar]
  33. McGeer P. L., Kawamata T., Walker D. G. Distribution of clusterin in Alzheimer brain tissue. Brain Res. 1992 May 8;579(2):337–341. doi: 10.1016/0006-8993(92)90071-g. [DOI] [PubMed] [Google Scholar]
  34. Michel D., Chabot J. G., Moyse E., Danik M., Quirion R. Possible functions of a new genetic marker in central nervous system: the sulfated glycoprotein-2 (SGP-2). Synapse. 1992 Jun;11(2):105–111. doi: 10.1002/syn.890110203. [DOI] [PubMed] [Google Scholar]
  35. Michel D., Chatelain G., Herault Y., Brun G. The long repetitive polypurine/polypyrimidine sequence (TTCCC)48 forms DNA triplex with PU-PU-PY base triplets in vivo. Nucleic Acids Res. 1992 Feb 11;20(3):439–443. doi: 10.1093/nar/20.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Michel D., Gillet G., Volovitch M., Pessac B., Calothy G., Brun G. Expression of a novel gene encoding a 51.5 kD precursor protein is induced by different retroviral oncogenes in quail neuroretinal cells. Oncogene Res. 1989;4(2):127–136. [PubMed] [Google Scholar]
  37. Murphy B. F., Davies D. J., Morrow W., d'Apice A. J. Localization of terminal complement components S-protein and SP-40,40 in renal biopsies. Pathology. 1989 Oct;21(4):275–278. doi: 10.3109/00313028909061073. [DOI] [PubMed] [Google Scholar]
  38. Mösch H. U., Graf R., Schmidheini T., Braus G. Three GCN4 responsive elements act synergistically as upstream and as TATA-like elements in the yeast TRP4 promoter. EMBO J. 1990 Sep;9(9):2951–2957. doi: 10.1002/j.1460-2075.1990.tb07487.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Ojeda F., Guarda M. I., Maldonado C., Folch H., Diehl H. Role of protein kinase-C in thymocyte apoptosis induced by irradiation. Int J Radiat Biol. 1992 May;61(5):663–667. doi: 10.1080/09553009214551471. [DOI] [PubMed] [Google Scholar]
  40. Palmer D. J., Christie D. L. The primary structure of glycoprotein III from bovine adrenal medullary chromaffin granules. Sequence similarity with human serum protein-40,40 and rat Sertoli cell glycoprotein. J Biol Chem. 1990 Apr 25;265(12):6617–6623. [PubMed] [Google Scholar]
  41. Rajotte D., Haddad P., Haman A., Cragoe E. J., Jr, Hoang T. Role of protein kinase C and the Na+/H+ antiporter in suppression of apoptosis by granulocyte macrophage colony-stimulating factor and interleukin-3. J Biol Chem. 1992 May 15;267(14):9980–9987. [PubMed] [Google Scholar]
  42. Saitoh T., Masliah E., Jin L. W., Cole G. M., Wieloch T., Shapiro I. P. Protein kinases and phosphorylation in neurologic disorders and cell death. Lab Invest. 1991 May;64(5):596–616. [PubMed] [Google Scholar]
  43. Singer V. L., Wobbe C. R., Struhl K. A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation. Genes Dev. 1990 Apr;4(4):636–645. doi: 10.1101/gad.4.4.636. [DOI] [PubMed] [Google Scholar]
  44. Smale S. T., Baltimore D. The "initiator" as a transcription control element. Cell. 1989 Apr 7;57(1):103–113. doi: 10.1016/0092-8674(89)90176-1. [DOI] [PubMed] [Google Scholar]
  45. Sylvester S. R., Morales C., Oko R., Griswold M. D. Localization of sulfated glycoprotein-2 (clusterin) on spermatozoa and in the reproductive tract of the male rat. Biol Reprod. 1991 Jul;45(1):195–207. doi: 10.1095/biolreprod45.1.195. [DOI] [PubMed] [Google Scholar]
  46. Tomei L. D., Kanter P., Wenner C. E. Inhibition of radiation-induced apoptosis in vitro by tumor promoters. Biochem Biophys Res Commun. 1988 Aug 30;155(1):324–331. doi: 10.1016/s0006-291x(88)81088-x. [DOI] [PubMed] [Google Scholar]
  47. Ucker D. S. Death by suicide: one way to go in mammalian cellular development? New Biol. 1991 Feb;3(2):103–109. [PubMed] [Google Scholar]
  48. Wasylyk C., Imler J. L., Wasylyk B. Transforming but not immortalizing oncogenes activate the transcription factor PEA1. EMBO J. 1988 Aug;7(8):2475–2483. doi: 10.1002/j.1460-2075.1988.tb03094.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Williams G. T. Programmed cell death: apoptosis and oncogenesis. Cell. 1991 Jun 28;65(7):1097–1098. doi: 10.1016/0092-8674(91)90002-g. [DOI] [PubMed] [Google Scholar]
  50. Williams T., Tjian R. Analysis of the DNA-binding and activation properties of the human transcription factor AP-2. Genes Dev. 1991 Apr;5(4):670–682. doi: 10.1101/gad.5.4.670. [DOI] [PubMed] [Google Scholar]
  51. Wilson M. R., Roeth P. J., Easterbrook-Smith S. B. Clusterin enhances the formation of insoluble immune complexes. Biochem Biophys Res Commun. 1991 Jun 28;177(3):985–990. doi: 10.1016/0006-291x(91)90635-k. [DOI] [PubMed] [Google Scholar]
  52. de Silva H. V., Stuart W. D., Park Y. B., Mao S. J., Gil C. M., Wetterau J. R., Busch S. J., Harmony J. A. Purification and characterization of apolipoprotein J. J Biol Chem. 1990 Aug 25;265(24):14292–14297. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES