Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cardiovasc Pathol. 2012 Feb 1;21(5):398–413. doi: 10.1016/j.carpath.2011.12.005

Fig. 9. Deletion of TNC gene up-regulates VCAM-1 expression in endothelial cells.

Fig. 9

(Panel A) Cultured endothelial cells isolated from the two mouse genotypes were analyzed by FACS using anti-VCAM-1 antibody. The cells were cultured on a collagen substrate and some cells were treated with 2.5 U/ml of TNFα for 4 hr. The cells were harvested and analyzed by FACA using anti-VCAM-1 antibody. (Panel B) The fluorescent-labeled RAW monocytic cells were added to unactivated (control) or TNFα-activated cells for 30 min. After several washes, the number of adherent cells was determined under the microscope. The data is the average of number of cells in 4 viewing fields.