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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Prostate. 2011 Nov 29;72(10):1140–1149. doi: 10.1002/pros.22463

Fig. 5. Heregulin-α regulates sPLA2-IIa promoter activity.

Fig. 5

LNCaP-AI (A) and LAPC4 (B) cells (105 cells/well) were seeded in a 12 well plate. Next day, the cells were transiently transfected with sPLA2-IIa(−800)-Luc (0.25 ug/well) overnight. The cells were then treated with Lapatinib (10 uM) and Bortezomib (5 uM) without or with EGF (100 ng/ml) or Heregulin-α (50 ng/ml) for 24 hours. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.