Table 1.

Primers, PCR products specific for the studied gene targets, and conditions for the multiplex PCR reaction

Studied targets	Primer sequences 5′–3′	T m [°C]a	Primers		PCR products [bp]
			Names	[μmol/L]b
S. agalactiae	TTT CAC CAG CTG TAT TAG AAG TA	55.3	Sag59	0.125	153
	GTT CCC TGA ACA TTA TCT TTG AT	55.3	Sag190
	(Ke et al. 2000)
Ia serotype	GG TCA GAC TGG ATT AAT GGT ATG C	64.0	Ia-F	0.25	521
	GTA GAA ATA GCC TAT ATA CGT TGA ATG C	64.0	Ia-R		1,826
	(Poyart et al. 2007)
III serotype	TCC GTA CTA CAA CAG ACT CAT CC	63.0	III-F	0.5	1,826
	AGT AAC CGT CCA TAC ATT CTA TAA GC	63.0	III-R
	(Poyart et al. 2007)
V serotype	GAG GCC AAT CAG TTG CAC GTA A	64.0	V-F	0.375	701
	AAC CTT CTC CTT CAC ACT AAT CCT	62.0	V-R
	(Poyart et al. 2007)
mefA/E gene	AGT ATC ATT AAT CAC TAG TGC	46.5	mefA	0.25	348
	TTC TTC TGG TAC TAA AAG TGG	48.5	mefE
	(Sutcliffe et al. 1996)
erm B gene	GGA AAG GTA CTC AAC CAA ATA A	46.5	ermB1	0.375	639
	CAT TTG TTA AAT TCA TGG CAA TGA	48.8	ermB2
	(Sutcliffe et al. 1996)
ST17 clone	ATA CAA ATT CTG CTG ACT ACC G	51.1	ST17S	0.125	210
	TTA AAT CCT TCC TGA CCA TTC C	51.1	ST17AS
	(Lamy et al. 2006)

^a T _m temperature specified by the manufacturer

^bConcentration of primers in the multiplex reaction mixture