Figure 6.

Immunofluorescent intensity quantification of the spinal cord dorsal horn reveals that AM1241 reduces the expression of the endocannabinoid degradative enzyme, MAGL but does not alter FAAH. (A, B) Compared to control rats, MAGL IR expression was increased ipsilaterally, with strong trends contralaterally in CCI-treated neuropathic rats that received i.t vehicle of AM1241, while spinal MAGL IR in CCI-treated rats given i.t. AM1241 was substantially reduced. (C, D) No changes in FAAH IR expression in ipsilateral and contralateral dorsal horn of either sham- or CCI-neuropathic rats given either i.t. vehicle or AM1241 was observed. All sections were 7 μm in thickness.