Fig. 3.

Phenotypes of the atx1 atx2 atxr7 triple mutant. (A) Representative plants of FRI-Col, FRI atx1-2 atx2-3, FRI atx1-2 atxr7-2, FRI atx1-2 atx2-3 atxr7-2 and Col (7 weeks old) grown in long days. At this stage, half of the FRI atx1-2 atx2-3 plants started to flower, but none of the FRI-Col plants had flowered. (B) Primary leaf number at flowering of FRI-Col, Col and atx multiple mutants grown in long days. Closed and open bars indicate rosette and cauline leaves, respectively. Error bars indicate the SD. (C) Real-time PCR was performed to analyze expression levels of FLC in FRI-Col, FRI atx1-2 atx2-3 atxr7-2 and Col. RNA was isolated from whole 10-day-old seedlings grown in continuous light. The averages of the results from three different biological replicates are shown. Each experiment was normalized to ACTIN7 (ACT7) expression. Error bars indicate the SE.