Fig. 4.

Phenotypes of atxr3 in a FRI-Col background. (A) Representative plants of FRI-Col and FRI atxr3-1 (12 weeks old) grown in long days. (B) Primary leaf number at flowering of FRI-Col and FRI atxr3-1 grown in long days. Closed and open bars indicate rosette and cauline leaves, respectively. The averages of the results from at least 12 plants are shown. Error bars indicate the SE. (C) Real-time PCR was performed to analyze the expression of FLC and the FLC-clade genes in FRI-Col (filled bars) and FRI atxr3-1 (open bars). Almost fully expanded rosette leaves that look vital and completely green were sampled from several 6-week-old plants. Plants had not flowered yet at this stage. The averages of the results from four different biological replicates are shown. The data from each experiment were normalized to ACT7 expression. Error bars indicate the SE.