Figure 4.

Examples of glycine- and ivermectin-mediated fluorescence and current responses in mutant GlyRs. Current responses (black) and fluorescence responses (red) were recorded simultaneously from Xenopus oocytes expressing indicated α1 GlyRs. (A) Fluorescence responses induced by glycine, but not ivermectin, in fluorescently labeled H201C mutant GlyRs. (B) Fluorescence responses induced by glycine and ivermectin in fluorescently labeled Q67C mutant GlyRs. (C) Fluorescence responses induced by glycine and ivermectin in fluorescently labeled Q67C, A288G, L233W triple-mutant GlyRs. These mutations convert ivermectin into an antagonist of glycine-gated currents. The figure is modified from Wang and Lynch (2012). Please refer to that paper for full experimental details.