Figure 6.

Pre-treatment with the CB₂ receptor agonist JWH-133 reduces tumour necrosis factor-α-mediated phosphorylation of ERK1/2 in human neutrophils. Neutrophils were pre-incubated for 2 h at 37°C in the presence or absence of different concentrations of JWH-133 (JWH). Then, without washing, cells were further stimulated for 7 min at 37°C in the presence or absence of control medium (CTL), cannabinoid vehicle (V), 1 μM JWH-133 (JWH), or 100 ng/mL tumour necrosis factor-α. (A) Representative western blot analysis of ERK1/2 phosphorylation. (B) Densitometric analysis of phospho-ERK1/2 (P-ERK1/2). Values were, respectively, normalized to amounts of total ERK1/2 and expressed as percentage of control medium (CTL), defined as 100%. Data are expressed as mean ± SD (n = 3).