Figure 2.
Hemopexin increases hemin export or extraction from cultured cortical cells. A) Medium 55Fe signal in cultures treated with 5 μM 55Fe-hemin alone as in Fig. 1, then washed and incubated in isotope-free MEM10 alone (Control) or MEM10 containing indicated concentrations of hemopexin (Hpx) or albumin (Alb) for 2 hours. B) Each bar represents the sum of signals in medium and cell lysate, 2 hours after 55Fe-hemin washout. *P<0.05, **P<0.01, **P<0.001 v. corresponding hemin+albumin condition, Bonferroni multiple comparisons test, n=6–15/condition.