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. Author manuscript; available in PMC: 2012 May 7.
Published in final edited form as: Sci Transl Med. 2011 Oct 12;3(104):104ra100. doi: 10.1126/scitranslmed.3002949

Fig. 1.

Fig. 1

High amounts of TH17 cells are found in sites of chronic diseases. Case numbers: blood (31), oral mucosa (6), spleen (5), tonsil (6), colon cancer (21), and colitis (12). (A) High numbers of IL-17+ cells were detected in oral mucosa tissues in patients with GVHD. Consecutive sections of oral mucosa biopsies were stained for IL-17 (brown) or CD3 (red). P < 0.001, acute versus chronic. (B) High percentages of TH17 cells were found in colitic colon and colon cancer tissues. Tissue single cells were stained for TH17 cell markers, analyzed by flow cytometry, and gated on CD3+ cells. (C and D) CD4+ and TH17 cells were found in colitic colon tissues. Colon tissues were stained for CD3 (green) (C). Absolute numbers of CD4+ and TH17 cells were quantified based on flow cytometry analysis. Results are expressed as the absolute numbers of CD4+ or TH17 per microgram tissue ± SEM (D). P < 0.001, colitic lesion (empty bars) versus adjacent tissue (filled bars). (E) Colon tissues were stained for CD3 and IL-17, and analyzed with a fluorescence microscope. Red, CD3; green, IL-17. DAPI, 4′,6-diamidino-2-phenylindole. (F to H) TH17 cells were found in different organs. Single cells were stained for TH17 cell markers and analyzed by flow cytometry. P < 0.001, tonsil and spleen as compared with blood.