Figure 1.

Experimental set-up for examining the impact of different intensities of green light (GL) on the regulation of cellular morphology in the chromatically adapting cyanobacterium Fremyella diplosiphon UTEX 481 wild-type (WT). A liquid culture of F. diplosiphon WT was initiated in BG-11 culture medium containing 20 mM HEPES (BG-11/HEPES) from a culture growing on a BG-11/HEPES plate under white fluorescent room light at room temperature (RT). Replicate liquid cultures at an absorbance at 750 nm (OD₇₅₀) of ∼0.1 were grown under GL at 10, 50, 75, or 100 μmol m⁻² s⁻¹ at 28°C with shaking at ∼175 rpm for 7 days. Cultures were then diluted to an OD₇₅₀ of ∼0.1 every 7 days for five consecutive dilutions, followed by dilution to OD₇₅₀ of ∼0.1 every 3 days. Cells were prepared for imaging after 5th, 8th, 17th, and 25th dilutions.