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Localization of FLAG-POF during interphase. P[yellow+ hsp70: FLAG-Pof] larvae were heat shocked, and their polytene chromosomes were stained with (A) DAPI and (B) anti-FLAG antibodies. The FLAG-POF protein is found exclusively on chromosome 4. (C) Merged DAPI and FLAG-POF images showing that POF decorates interbands preferentially.
