Figure 4.
POF localization on translocation chromosomes. T(1;4)wm5 males stained with DAPI (A, C, E, and G) and anti-POF (B, D, F, and H). The distal X chromosome portion translocated to proximal chromosome 4 is indicated by dX, and the proximal X region is labeled pX. The distal 4 portion translocated to X:3C is labeled d4, whereas normal 4 is labeled 4. (A–D) Only the normal 4 is stained when 4 and d4 are not paired. (E–H) Staining spreads in trans when 4 and d4 are paired. (I–L) T(3;4)e chromosomes stained with DAPI (I and K) and anti-POF (J and L). Normal fourth chromosome and proximal chromosome 4 with 3L translocated to the tip are marked 4 and p4, respectively. Translocation breakpoints and cytology are indicated.