Fig. 9.

In vivo fluoresence images of a ganglion cell expressing YFP. (a-c) Individual images from three of the focuses, at focus steps of 11.6 μm. Each image at an individual focus step was a registered image average of 500 frames. (d) Maximum intensity projection image generated from 5 separate in vivo images taken at focus steps of 5.8 μm. Confocal pinhole diameter was 3.9 Airy disks. All images were contrast stretched identically to preserve their relative brightness. Scale bar: 20 µm.