Fig. 11.

Characterization of the in vivo resolution using an image stack of a fluorescent ganglion cell. (a) Maximum projection image for a focus stack. (b) One individual focus from the focus stack. Transverse cross section on a typical dendrite labeled in yellow is plotted as an example in (d). (c) An individual focus image 11.6 μm shallower than the focus in (b). Arrow indicates measurement position for a typical axial cross section shown in (e). (d) and (e) are the characterization of the in vivo transverse and axial resolution, respectively. Circle data points: in vivo measurement. Solid black line: spline fit to in vivo measurement data. Solid gray line: theoretical diffraction-limited axial PSF. Scale bar: 20 µm.