Fig. 6.

Effect of cucurbitacin B on cell morphology. a Caki-1 cells were treated with or without cucurbitacin B (200 nM) for 1 h in the presence or absence of 10 mM NAC. b Caki-1 cells were treated for 1 h with 200 nM cucurbitacin B followed by paraformaldehyde fixation and permeabilization with Triton X-100 before visualization of polymerized actin using fluorescein-phalloidin. c ACHN or Caki-1 cells were treated for 1 h with cucurbitacin B (200 nM), solubilized and subjected to western blot analysis using an antibody against phosphopaxillin