Table 4.
Inhibition constants of ANF, ellipticine and furafylline for EROD and MROD in the microsomes from castrated and entire male pigs.
| Inhibitor | Ki, μM
|
|||
|---|---|---|---|---|
| EROD | MROD | |||
| Entire male pigs | Castrated pigs | Entire male pigs | Castrated pigs | |
| ANF | 0.031 | 0.096 | 0.014 | 0.018 |
| Ellipticine | 0.017 | 0.021 | 0.024 | 0.019 |
| Furafylline | no* | no* | 102.7 | 37.6 |
ANF: α-naphthoflavone; EROD: ethoxyresorufin O-deethylation; MROD: methoxyresorufin O-demethylation. The inhibitior constants Ki were calculated using nonlinear regression analysis with GraphPad Prism program 4.0 kinetic software.
*
No inhibition was observed within the range of substrate concentrations used.