Figure 4.

(A) Representative sections of nuclear DNA fragmentation staining performed by terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) in the CA₁ region of the hippocampus from rats that randomly received 5-HD (20 mg/kg, intravenously) or DIAZ (5 mg/kg, intravenously) after 90 minutes of ischemia and 72 hours of reperfusion (Con). In the presence and absence of 5-HD or DIAZ pretreatment, or with a combination of DRIPost (three cycles of 5 minutes occlusion/reperfusion at 6 hours after reperfusion) and 5-HD or DIAZ. (a) Control (Con); (b) 5-HD; (c) DIAZ; (d) DRIPost; (e) DRIPost+5-HD; (f) DRIPost+DIAZ. (B) Quantitative analysis of the number of TUNEL-positive neurons in the CA₁ region of the hippocampus. Data are presented as the mean±s.d. Scale bar, 20 μm. ^#P<0.05 versus Con group. ^*P<0.05, versus DRIPost group. DIAZ, diazoxide; 5-HD, 5-hydroxydecanote.