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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Lab Invest. 2011 Jun 20;91(9):1383–1395. doi: 10.1038/labinvest.2011.86

Figure 2.

Figure 2

NanoCurc™ prevents carbon tetrachloride (CCl4) associated liver injury and inflammation. The mice were injected with either phosphate-buffered saline (PBS), void nanoparticles (Poly) or NanoCurc™ (NC) injections along with CCl4 according to the treatment protocol in Materials and Methods. (a) Liver damage was determined by measuring serum alanine aminotransferase (ALT) activity (expressed in Sigma Frankel units) and (b) intrahepatic tumor necrosis factor (TNF)-α was determined by enzyme-linked immunosorbent assay (ELISA). (c and d) Relative interleukin (IL)-6 and TNF-α mRNA expression was determined with real-time reverse transcription-polymerase chain reaction (RT-PCR) using the ΔΔCt method with histone 2AZ and hprt serving as a reference genes. PBS-negative control liver tissue was set to relative expression level of 1.0. Data were expressed as mean ± s.e.m., *P < 0.05, **P < 0.001, t-test, from two experiments.