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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Lab Invest. 2011 Jun 20;91(9):1383–1395. doi: 10.1038/labinvest.2011.86

Figure 3.

Figure 3

NanoCurc™ inhibits carbon tetrachloride (CCl4)-induced liver injury and fibrosis. Mice were treated with intraperitoneal control (a, c) or NanoCurc™ (b, d) injection and CCl4 according to the treatment protocol in Materials and Methods. (a, b) Methacarn-fixed liver tissues were stained with Sirius Red, which highlights collagen deposition in dark red. Representative photomicrographs (× 10 objective). (c, d) The extent of fibrosis was quantitatively determined using Threshold Image Analysis to highlight fibrosis (orange color) in 10 randomly selected photomicrographs (× 10 objective) after conversion to 8-bit gray scale. (e) The percentage of fibrosis calculated as the area of fibrosis divided by the total image area multiplied by 100. (f) Hydroxyproline assay was carried out to quantify the collagen content. Data were expressed as mean ± s.e.m.,*P < 0.05, **P < 0.001, t-test, from two experiments.