Fig. 1.
Expression of calcium-activated, large conductance potassium (BKCa) channel in rat intestinal enterocytes. Top: Western blot of total membrane and crude mitochondrial fractions from isolated mouse ileal enterocytes (see methods), each probed for the α-subunit of BKCa channel, mitochondrial porin (VDAC, outer membrane marker for mitochondria), and E-cadherin (plasma membrane protein used as marker for the total membrane fraction). Bottom, A: immunostaining of BKCa α-subunit protein in enterocytes of rat ileal mucosa. Bottom, B: negative controls were performed by using BKCa-α primary antibody that had been preabsorbed by use of a control peptide. Scale bar = 20 μm.