Fig. 6.

Colonic mucosal cytokine mRNA expression. RNA was isolated from colonic mucosal scrapings from mice following exposure to carcinogen and 3 subsequent cycles of DSS. mRNA levels were determined by quantitative RT-PCR, and expression of each gene of interest was normalized to ribosomal 18S expression. Relative expression levels were analyzed for IL-6 (A), IFN-γ (B), IL-17A (C), IL-17F (D), IL-23 (E), and IL-23R (F). Data were analyzed by 2-way ANOVA (main effects: diet and genotype), and significance was at the level of P < 0.05. For all data sets, P (interaction) was not significant (P > 0.05); however, the outcome from each main effect is shown. Values are means ± SE.