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T-84 cells were nucleoporated with IFN-γ reporter construct pGAS-Luc (GAS) and treated with 100 U/ml IFN-γ for 5 h with and without 50 μM curcumin (administered 20 min prior to the addition of IFN-γ). Luciferase activity was measured with cell extract by using firefly luciferase assay kit and the results were calculated as relative light units (RLU) per μg protein. *Statistically significant difference (P ≤ 0.05) between reporter activity (Student's t-test; n = 3).
