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Real-time RT-PCR analysis of the effects of curcumin on IFN-γ-induced MHC-II gene expression in T-84 cells. Cells were stimulated with 100 U/ml IFN-γ for 24 h with or without curcumin (25–75 μM; administered 20 min prior to the addition of IFN-γ). TBP was used as a reference gene. Different symbols next to bars indicate statistical significances (P ≤ 0.05) between groups [ANOVA followed by Fisher protected least significant difference (PLSD) post hoc test; n = 4].
