Fig. 6.

Western blot analysis of the effects of IFN-γ and curcumin on phosphorylation of Stat1 (pTyr⁷⁰¹), Jak1 (pTyr¹⁰²²), and SHP-2 (pTyr⁵⁴²). Respective total protein was analyzed as loading control. Cells grown in 24-well plates were harvested in 100 μl hot Laemmli sample buffer, and 20 μl was used for electrophoresis. A: T-84 cells were pretreated with indicated concentrations of curcumin for 20 min prior to stimulation with IFN-γ (100 U/ml) for 2.5 or 5 min. B: inhibition of Stat1 activation in in YAMC cells. YAMC cells were treated with curcumin (10–75 μM) for 20 min as indicated and/or treated with murine (m)IFN-γ (100 U/ml) for 10 min. C: inhibition of Jak1 activation in T-84 cells. Cells were treated in a way analogous to A, but with 10-min stimulation with IFN-γ. D: effects of curcumin and IFN-γ on SHP-2 activation in T-84 cells. Cells were pretreated with 50 μM curcumin for 20 min prior to 10-min stimulation with IFN-γ (100 U/ml).