Fig. 2.

LPC induces caspase-dependent apoptosis in hepatocytes. A: Huh-7 cells were incubated with LPC (85 μM) in the presence of the pan-caspase inhibitor Q-Val-Asp-OPh (QVD-OPh; 10 μM) for 16 h. Vehicle-treated cells (Veh) were used as controls. Apoptotic nuclei were counted after DAPI staining, and caspase 3/7 catalytic activity was measured using a fluorogenic assay. B: mouse primary hepatocytes (MPH) or human primary hepatocytes (HPH) were treated with LPC for 6 or 3 h, respectively. The concentrations of LPC used were 75 μM for MPH and 85 μM for HPH. Apoptosis was assessed as in A. All data are means ± SE for 3 experiments. *P < 0.01.