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. 2011 Oct 13;302(1):G77–G84. doi: 10.1152/ajpgi.00301.2011

Fig. 3.

Fig. 3.

LPC induces JNK phosphorylation, and JNK inhibition and glycogen synthase kinase-3 (GSK-3) inhibition reduce c-Jun phosphorylation in hepatocytes. A: immunoblot analysis was performed for phosphorylated (phospho-) and total JNK. Whole cell lysates were prepared from Huh-7 cells incubated with LPC (85 or 42.5 μM) for 8 h or from MPH incubated with LPC (85 μM) for 4 h. B: immunoblot analysis was performed for phosphorylated and total JNK. Whole cell lysates were prepared from Huh-7 cells incubated with LPC (85 μM) for the indicated times. C: immunoblot analysis was performed for phosphorylated and total c-Jun. Whole cell lysates were prepared from Huh-7 cells incubated with LPC at 85 μM in the presence of the JNK inhibitor SP600152 (SP) at 50 μM for 8 h or from MPH incubated with LPC (85 μM) in the presence of SP (50 μM) for 4 h. D: immunoblot analysis was performed for phosphorylated and total c-Jun. Whole cell lysates were prepared from Huh-7 cells incubated with LPC (85 μM) in the presence of the GSK-3 inhibitor GSK-IX (2 μM) for 8 h.