Fig. 6.

LPC-induced Bax activation is JNK and GSK-3 dependent, and LPC-induced apoptosis is mediated by PUMA. A: Huh-7 cells or MPH were incubated with LPC (85 μM) for the indicated times. Representative images of 3 independent experiments are depicted. 6A7-immunoreactive cells, indicative of an activating conformational change in Bax, were quantified in 10 random ×40 objective fields for each condition, and the percentages of 6A7-positive vs. total cells are shown in bar graphs. B: Huh-7 cells or MPH were incubated with LPC (85 μM) in the presence of the JNK inhibitor SP (50 μM) or the GSK-3 inhibitor GSK-IX (2 μM) for 8 or 3 h, respectively. Hepatocytes were analyzed for Bax 6A7 immunoreactivity as described in A. C: MPH were isolated from either WT or Puma^−/− mice and treated with LPC (75 μM) for 6 h. Vehicle-treated cells were used as controls. All data are means ± SE for 3 experiments. *P < 0.01.