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. 2012 Jan 24;20(5):1002–1013. doi: 10.1038/mt.2011.298

Figure 2.

Figure 2

Sensitivity of deoxyCytidine Kinase (dCK)-transduced cells to prodrug treatment in vitro. Transduced and fluorescence-activated cell sorting (FACS)-enriched (a,b) Jurkat, (c,d) Molt-4, and (e,f) U87mg cells were treated with increasing concentrations of two thymidine-based nucleoside analogues, 0.1 µmol/l–1 mmol/l bromovinyl-deoxyuridine (BVdU) (a,c,e) and 0.1 µmol/l-10 mmol/l L-deoxythymidine (LdT) (b,d,f), in culture over a period of 5 days. After 5 days, viability was assessed by the MTS assay. Corresponding mean viability is plotted for each experiment. Cells were either nontransduced (closed diamonds), or transduced with either dCK.WT (closed squares), dCK.DM (open circles), or dCK.DM.S74E (open triangles). Data shown for each group were normalized to corresponding nontreated controls (error bars represent SE of the mean; each analysis was performed at least in triplicate; *indicates statistically significant difference compared to nontreated control, P < 0.01).