Figure 3.

Detection of knockdown and overexpression in primary CD₄⁺ T-cells. (a) Analysis of protein expression in transgenic primary CD₄⁺ T-cells and in wild-type (WT) cells. Equal loading was controlled by α-tubulin. (b) LEDGF/p75 KD (white bar) compared to WT (black bar) measured by quantitative reverse transcriptase (QRT)-PCR. (c) LEDGF_325–530 (white bar, horizontal lines) and LEDGF_325–530D366N (gray bar, horizontal lines) overexpression compared to WT (black bar) measured by QRT-PCR. mRNA levels were normalized for β-actin mRNA. The data are represented as mean + SD of at least three measurements. LEDGF/p75, lens epithelium-derived growth factor; KD, knockdown; WT, wild type.