Figure 3.

BRA-1 associates with the DAF-1 type I receptor through the conserved C-terminal region in mammalian cells. (A) Schematic diagram of the structure of BRA-1 deletion mutations. (B) COS7 cells were transiently transfected with HA-tagged BRA-1, BRA-1ΔN, and BRA-1ΔC and GST alone or GST-DAF-1. Cell lysates were subjected to the GST pull-down procedure and then immunoblotted using an anti-HA antibody (Top). Expression of BRA-1-HA, BRA-1ΔN-HA, BRA-1ΔC-HA, GST, and GST-DAF-1 was measured by anti-HA or anti-GST immunoblotting of aliquots from cell lysates (Middle or Bottom).