Figure 3.

The distance values returned in the final output spread-sheet are illustrated here with distances being calculated relative to the primers and exon involved. α) Primer start to exon start is calculated from the 5' end of the forward primer to the 5' end of the exon. β) Exon finish to primer finish is calculated as the distance between the 3' end of the exon to the 5' end of the reverse primer. In the case of exons that include the start/stop of the coding sequence, these values are used to replace the exon 5'/3' ends, respectively.