TABLE 1.

Overall parameters of PabNucS, PabPCNA, and the complex PabNucS-PCNA determined by SAXS

Proteins	Mr	Concentration	Rga	MassGuinierb	Quality of the fit	RgPoroda	MassPorodb	RgP(r)a	Dmaxc	Estimate	MassP(r)ab	Rgatomica	Dmaxatomicc	Best χ2	NSD between 10 models
		mg ml−1	Å	kDa	%	Å	kDa	Å	Å		kDa	Å	Å
NucS	59.2	4.31	35.0	60.7	86	35.6	59.2	35.6	111	0.908	55.0	34.5	111.8	0.7912	1.510
NucS + DNA	59.2	3.56	35.0	63.2	91	35.7	62.4	35.6	111	0.935	55.5	34.6	112.4	0.9625	1.717
PCNA	86.4	5.3	33.5	84.7	85	33.7	84.1	34.1	99	0.968	82.5	33.6	95.6	0.8060	1.157
NucS + PCNA	145.6	4.6	38.7	148.9	89	38.8	132.8	39.4	130	0.889	137.5	39.9	131.2	1.242	0.833

^a R_g, R_g^Porod, R_g^P(r), and R_g^atomic given by the Guinier approximation, deduced from the Porod calculation, derived from the distance distribution function, and calculated for the final atomic model using the program CRYSOL, respectively.

^b Mass^Guinier, Mass^Porod, Mass^P(r), mass of the protein given by the Guinier approximation, deduced from the Porod calculation, derived from the distance distribution function, and calculated for the final atomic model using the program CRYSOL, respectively.

^c D_max and D_max^atomic correspond to the values deduced, respectively from the distance distribution function and the final model. The programs developed by Svergun and co-workers (65) minimize the normalized discrepancy function χ².