FIGURE 5.

The Dyn2-Nup159 pep2 interaction is mediated by an extensive interaction network that recognizes 10 contiguous Nup159 residues, dually conferring specificity and substrate plasticity. A and B, close-up of residues involved in the Dyn2-Nup159 pep2 interaction. Secondary structure elements are shown as in Fig. 2A, with specific residues that mediate the Dyn2-Nup159 pep2 interaction shown in stick format and their corresponding hydrogen-bonding network shown with dashed lines. C, interaction matrix, showing the contact networks formed between Dyn2 protomers A and C with Nup159. Secondary structure elements and protomer designation are indicated along matrix axes. Backbone-backbone, backbone-side chain, side chain-side chain, and van der Waals interactions are indicated in blue, pink, red, and gray, respectively, and correlate with distances less than or equal to 3.5 (hydrogen bonds) and 4.5 Å (van der Waals contacts).